Streptococcus Colonial Growth of Dental Plaque Inhibition Using Flavonoid Extract of Ants Nest (Myrmecodia pendans): An in Vitro Study

Objective: To know the activity of resistance of flavonoid content in ant nest plant in decreasing the number of colonies S. mutans oral cavity of children as a medic herbal material. Material and Methods: The subjects were plaque sample of 20 children aged 7-12 years. Research begins by making toothpaste from ant nest extract. Samples of children's dental plaque were inserted into BHIB media, after which incubated for 24 hours, 1/10 dilution with BHIB media three times, followed by TYC media planting and incubation of anaerob with temperature 370C for 48 hours. After that then count the number of colonies of S. mutans. Results: On ethyl acetate extract of ant nest incubated at room temperature with concentration 20%, 40%, 60%, 80%, 100% obtained a decrease from each treatment amount of Streptococcus mutans colony on TYC media with median value of each treatment was 89, 67, 64, 61, 59 and 51 for the ethyl acetate fraction, and 62, 61, 60, 59, 49 at the ethanol fraction. There was no significant difference between the six concentration groups (p>0.05). Conclusion: Flavonoids extract of ant nest plants have growth barrier on Streptococcus mutans bacteria, the greater the concentration given the greater the number of S. mutans colony.

Anti-Cancer and Anti-Proliferation Activity of Ethyl Asetat Extract From Ant Nest (Myrmecodia pendans) in Burkitt's Lymphoma Cancer Cells

Objective: To determine the activity of anti-cancer and anti-proliferation of ethyl acetate fraction of ant nest plants (Myrmecodia pendans) in Burkitt's Lymphoma cancer cells. Material and Methods: The study was conducted in a pure laboratory experimental method using Burkitt's Lymphoma cancer cell culture. Gradual research begins with the determination, extraction and fractionation of ant nest plants, to test for proliferation barriers. Data analysis using two-way ANOVA followed by Post Hoc LSD test with a significance level of 95%. Pearson correlation test was conducted. Results: The results of testing the inhibition of Burkitt's Lymphoma cell proliferation with ethyl acetate extract treatment showed that there was inhibition of cell growth based on the concentration given, starting from the lowest concentration of 15.625 µg/mL. Likewise, the incubation time factor of 24, 48, and 72 hours showed that the longer the incubation time, the greater the inhibition of cell growth. Antiproliferation analysis of flavonoid ethyl acetate extract based on concentration and incubation time on absorption of optical density Burkitt's Lymphoma was statistically significant (p = 0.00). Conclusion: Ant nest ethyl acetate extract has the effect of proliferation inhibition on Burkitt's lymphoma cells.

Analysis of Risk Factors of Biopsychosocial with Early Childhood Caries (ECC) in Indonesian Pre-School Children

Objective: To describe the Early Childhood Caries (ECC) in Indonesian preschool children based on biopsychosocial risk factors. Material and Methods: This research is an analytic observational with a cross sectional design toward 506 preschool children aged 2-6 years at East Luwu Regency, Indonesia. The biopsychosocial data studied were gender, ethnicity, personal hygiene, child psychology, age, nutritional status, and dental and oral health behavior. These data were obtained through a questionnaire and clinical examination (nutritional status). Multiple linear regression analysis was performed. The statistical tests were performed at the significance level of 0.05. Results: Characteristics of respondents based on parents' origin, found that the majority of respondents were parents from Bugis tribes (68.4%) with ECC frequency of 72.2%. Regarding the visit to the dentist, 12.6% were afraid of the dentist, with ECC frequency of 71.9%. Most children had normal nutritional status (67%) with ECC frequency of 69.3%. Biopsychosocial factors, which significantly affect the Early Childhood Caries, were parents' tribe (p=0.022) and age (p=0.011). There was an association between ECC with brushing teeth before going to bed (p=0.049), use of mouthwash (p=0.002) and consumption of snacks (p<0.001). Conclusion: Biopsychosocial factor indicators are affect the Early Childhood Caries towards Indonesian preschool children.

Pulse Rate Change After Childhood Anxiety Management with Modeling and Reinforcement Technique of Children's Dental Care

Objective: To know the influence of handling child anxiety with modeling and reinforcement technique on changes in pulse rate in dental and oral care. Material and Methods: The sample consisted of 53 children aged 6-12 years who first came to pedodontic clinic in dental and oral hospital education of Hasanuddin University. Anxiety level was measured using objective parameters, measurement of radial pulse. Measurement of anxiety level was done before and after modeling and reinforcement was given through three treatments: I: dental models; II: game and III: video modeling and reinforcement. Data analysis was done using the Kolmogorov-Smirnov test and Friedman test. Results: There was a decrease before and after modeling and reinforcement. Before intervention, the average pulse rates in treatment I, II and III were 90.79, 88.00 and 88.38, respectively. After intervention, the average decrease in pulse rate of five beats per minute seen in the first treatment (85.15), a decrease of seven beats per minute on the second treatment (81.98), while in treatment III the average decrease was eight beats per minute (80.19) (p<0.001). Conclusion: Modeling and reinforcement technique effectively reduces children's anxiety levels for dental and oral care.

Solution Inhibitory of Ant Nest (Myrmecodia Pendans) Extract on the Formation of Plaque in Children with Early Childhood Caries

Abstract Objective: To evaluate the effectiveness of gargling the solution of ant nest extract against plaque formation in children who experience Early Childhood Caries (ECC) when used as a mouthwash. Material and Methods: This study uses a pretest-posttest design with a control group. Thirty children with ECC, which were divided into three groups, namely the treatment group which was given a solution of extracts of ant nests concentrations of 5% and 10% and the control group gargling Aquades. Plaque scores were measured using the Greene and Vermillon method before and after the intervention. The paired t-test was used, with a significance level of 0.05 Results: Significant differences were found before and after the treatment of ant nest extract (r<0.05). The increase in OHI-S mean in the control group before treatment was 2.41 ± 0.72 and after treatment 2.53 ± 0.69 while in the treatment group there was a decrease in the OHI-S average in the group of 5% before treatment 2.66 ± 0.90 and after treatment 2.29 ± 0.95 and group concentration of 10% before treatment 2.51 ± 0.89 and after treatment 1.82 ± 0.75 Conclusion: The distribution of ant nest extract solution extract with the concentration of 5% and 10% would be more effective with ant nest concentration of 10% where it can inhibit dental plaque formation, and there is a significant difference in mean plaque scores between the control group and the treatment groups.

Determinant Factors of Dental Caries in Indonesian Children Age 8-12 Years

Objective: To evaluate the determinant factors of dental caries in Indonesian children aged 8-12 years. Material and Methods: This study was observational and analytical with a cross-sectional design. A total of 114 children were selected using simple random sampling technique. Primary data was obtained from a questionnaire about tooth brushing habits, the use of toothpaste containing fluoride and cariogenic eating habits and data of dental caries obtained from direct observed by a dentist. Univariat data were analyzed using descriptive and bivariate data were analyzed using Chi-square tests. Results: The results obtained by 44.8% fair category of children who have toothbrushing habits, use of toothpaste containing fluoride was 100%, and 71% middle category children who have cariogenic eating habits. The most consumed cariogenic food was ice cream with 76.3% participants consumed it one to three times a week. The most eaten cariogenic foods every day in this sample were syroup/pop ice soft drink (42.1%). No significant association between gender and dental caries incidence was observed (p>0.05). Conclusion: There is significant association between tooth brushing habits with dental caries in Indonesian children aged 8-12 years at primary school in district of East Luwu Regency Tomoni.